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J. Biochem, 2004, Vol. 136, No. 3 283-291
© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

Characterization of a Polysaccharide Deacetylase Gene Homologue (pdaB) on Sporulation of Bacillus subtilis

Tatsuya Fukushima1, Taichi Tanabe1, Hiroki Yamamoto1, Shigeo Hosoya2, Tsutomu Sato2, Hirofumi Yoshikawa3 and Junichi Sekiguchi1,*

1 Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567; 2 International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509; and 3 Department of Bioscience, Tokyo University of Agriculture, Sakuragaoka, Tokyo 156-8502

The predicted amino acid sequence of Bacillus subtilis ybaN (renamed pdaB) exhibits high similarity to those of several polysaccharide deacetylases. Northern hybridization analysis with sporulation sigma mutants indicated that the pdaB gene is transcribed by E{sigma}E RNA polymerase and negatively regulated by SpoIIID. The pdaB mutant was deficient in spore formation. Phase- and electron microscopic observation showed morphological changes of spores in late sporulation periods. The pdaB spores that had lost their viability were empty. Moreover, GFP driven by the promoter of the sspE gene was localized in the forespore compartment for the wild type, but was localized in both the mother cell and forespore compartments for phase-gray/dark forespores of the pdaB mutant. This indicates that GFP expressed in the forespores of the mutant leaks into the mother cells. Therefore, PdaB is necessary to maintain spores after the late stage of sporulation.

* To whom correspondence should be addressed. Tel: +81-268-21-5344, Fax: +81-268-21-5345, E-mail: jsekigu{at}giptc.shinshu-u.ac.jp


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