© 2004 The Japanese Biochemical Society
MOLECULAR BIOLOGY |
Activation of SoxR-Dependent Transcription in Pseudomonas aeruginosa
The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047
The SoxR protein of Escherichia coli responds to redox signals by activating the transcription of soxS, which encodes another transcription activator that directly stimulates oxidative stress genes. In contrast, Pseudomonas aeruginosa has an open reading frame (ORF) encoding a putative protein homologous to E. coli SoxR, but not to SoxS. Instead of a soxS homolog, ORFs encoding an unknown hypothetical protein and soxR are arranged divergently with their 5' ends separated by a 78 bp region containing a sequence homologous to the SoxR-binding soxS promoter. In this study, we report the overproduction and purification of SoxR from P. aeruginosa to investigate the mechanism of gene activation by SoxR. The spectroscopic properties of the purified SoxR protein indicate that it contains a redox active iron-sulfur [2Fe-2S] cluster. Redox titration of the SoxR protein revealed a midpoint potential of –290 mV. The SoxR protein specifically binds a fragment of the SoxS promoter–like region in a concentration-dependent fashion, as shown by both gel mobility shift and fluorescence polarization assays. The purified SoxR stimulates the in vitro transcription of the gene encoding the hypothetical protein in P. aeruginosa. This activity was lost following reduction of the SoxR [2Fe-2S] clusters. The levels of mRNA in the hypothetical protein increased in paraquat-treated cells. These results indicate that P. aeruginosa SoxR is a direct transcriptional activator of the hypothetical protein, and suggest that SoxR proteins may play multiple regulatory roles as a transcription factor in addition to its protective role in oxidative stress.
* To whom correspondence should be addressed. Tel: +81-6-6879-8502, Fax: +81-6-6876-3287, E-mail: kobayasi{at}sanken.osaka-u.ac.jp
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