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Journal of Biochemistry 2004 136(6):825-830; doi:10.1093/jb/mvh192
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© 2004 The Japanese Biochemical Society

MOLECULAR BIOLOGY

New DNA-Binding Activity of Rat Mitochondrial Transcription Termination Factor (mTERF)

Ascensión Prieto-Martín1, Julio Montoya1 and Francisco Martínez-Azorín1,2,*

1 Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, Miguel Servet 177, E-50013 Zaragoza, Spain; and 2 Departamento de Bioquímica (B-19), Facultad de Medicina (CSIC-UAM), Universidad Autónoma de Madrid, Arturo Duperier 4, E-28029 Madrid, Spain

The molecular mechanisms involved in the regulation of the balance between rRNA and mRNA in mitochondria are poorly understood. The mitochondrial transcription termination factor (mTERF) was highlighted as a potential transcription-control point. In this study, rat mTERF has been expressed in vitro and in Escherichia coli. The mature protein, in addition to the expected specific DNA–binding capacity for the sequence required for termination, has a new DNA-binding activity, and is able to bind to rat mitochondrial promoter region. This finding suggests communication between transcription initiation and termination regions. However, the results of a competition experiment argue against the formation of a complex between rat mTERF and the termination probe and promoter probe simultaneously, although it remains to be investigated whether another factor(s) might be involved in this interaction. In addition, recombinant human mTERF is also able to bind to human mitochondrial promoter region.

* To whom correspondence should be addressed at: Departamento de Bioquímica (B-19), Facultad de Medicina (CSIC-UAM), Universidad Autónoma de Madrid, Arturo Duperier 4. E-28029 Madrid, Spain. Phone: +34 91 497 5408, Fax: +34 91 585 4401, E-mail: fazorin{at}iib.uam.es


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