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Journal of Biochemistry 2005 137(1):33-39; doi:10.1093/jb/mvi007
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© 2005 The Japanese Biochemical Society

BIOCHEMISTRY

Expression Cloning of a Variety of Novel Protein Kinases in Lotus japonicus

Isamu Kameshita1,*, Tetsuyuki Nishida1, Satoko Nakamura1, Yasunori Sugiyama1, Noriyuki Sueyoshi1, Yosuke Umehara2, Mika Nomura1 and Shigeyuki Tajima1

1 Department of Life Sciences, Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa 761-0795; and 2 National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602

To investigate protein kinases expressed in Lotus japonicus, a cDNA expression library of the root-nodule of L. japonicus was immunologically screened with monoclonal antibodies directed to a highly conserved region in protein serine/threonine kinases (Ser/Thr kinases). Among 178 positive clones obtained from the {lambda}ZAPII cDNA library, 164 clones were found to encode novel proteins possessing the subdomain VIB sequences characteristic of Ser/Thr kinases. By phylogenetic analysis on the basis of deduced amino acid sequences, the isolated clones could be classified into five different families of Ser/Thr kinases : the SnRK family, GSK-3 family, Ndr kinase family, Ark family, and receptor kinase family. These results suggest that this expression cloning using the kinase-specific antibodies will provide new clues for investigations of a wide variety of known and novel protein kinases in higher plants.

* To whom correspondence should be addressed. Tel: +81-87-891-3120, Fax: +81-87-891-3120, E-mail: kamesita{at}ag.kagawa-u.ac.jp


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