© 2005 The Japanese Biochemical Society
Regular Paper |
Normal Human Sera Contain Bactericidal IgG That Binds to the Oligosaccharide Epitope Expressed within Lipooligosaccharides of Neisseria gonorrhoeae
Department of Biochemistry & Biotechnology, Tottori University, Tottori 680-8553
* To whom correspondence should be addressed. Tel: +81-857-31-6751, Fax: +81-857-31-5347, E-mail:yamasaki{at}muses.tottori-u.ac.jp
Although more than several investigators reported the presence of antibodies in normal human sera (NHS) that bind to lipooligosaccharide (LOS) of Neisseria gonorrhoeae, the specificities of those antibodies were not fully characterized. To identify anti-LOS antibodies in NHS, we used LOS from a serum-sensitive strain, JW31R, as an affinity ligand and purified IgG from NHS that bound to JW31R LOS. The affinity purified IgG (AP-IgG) binds to the oligosaccharide (OS) moiety of both the ligand LOS and its truncated form, 15253 LOS. Lipid A could be essential for maximum expression of the carbohydrate epitope that resides on 15253 OS. We also found that AP-IgG is capable of killing a serum-sensitive strain JW31R. The present work provided direct evidence that NHS contain bactericidal antibodies specific for a site close to the inner core OS expressed on gonococcal LOS. The present results not only show that anti-LOS antibodies specific for the inner core OS could play a major role in our defense against gram-negative bacteria. But also they demonstrated that such core OS or a nearby site could be utilized as possible targets for vaccine development against microbial infections.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. M. Estabrook, G. A. Jarvis, and J. McLeod Griffiss Affinity-Purified Human Immunoglobulin G That Binds a Lacto-N-Neotetraose-Dependent Lipooligosaccharide Structure Is Bactericidal for Serogroup B Neisseria meningitidis Infect. Immun., February 1, 2007; 75(2): 1025 - 1033. [Abstract] [Full Text] [PDF]
|
|