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Journal of Biochemistry 2005 137(5):601-606; doi:10.1093/jb/mvi076
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© 2005 The Japanese Biochemical Society

Regular Paper

Expression and Characterization of a Baseplate Protein for Bacteriophage Mu, gp44

Daisuke Kitazawa1,*, Shigeki Takeda1,{dagger}, Yasuhiro Kageyama1, Masashi Tomihara1 and Harumi Fukada2

1 Department of Nano-Material Systems, Graduate School of Engineering, Gunma University, 1-5-1 Tenjin-cho, Kiryu, Gunma 376-8515; and 2 Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531

{dagger} To whom correspondence should be addressed. Tel/ Fax: +81-277-30-1434, E-mail: stakeda{at}bce.gunma-u.ac.jp

The gene product of gene 44 of Mu phage (gp44) is an essential protein for baseplate assembly and has been designated as gpP, a traditional genetic assignment. The function of gp44 during the assembly or infection process is not known. In the present study, we purified the recombinant gp44 and characterized it by analytical ultracentrifugation and differential scanning microcalorimetry. The results indicate that gp44 forms a trimer comprising a complex consisting of the 42 kDa and 40 kDa subunits that had been cleaved in the C-terminal region. Thermodynamic analysis also suggested that the C-terminal region forms a flexible domain.

* Present address: Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577.


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