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Journal of Biochemistry 2005 137(6):721-729; doi:10.1093/jb/mvi090
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© 2005 The Japanese Biochemical Society

Regular Paper

Design, Expression and Solid-State NMR Characterization of Silk-Like Materials Constructed from Sequences of Spider Silk, Samia cynthia ricini and Bombyx mori Silk Fibroins

Mingying Yang and Tetsuo Asakura*

Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588

* To whom correspondence should be addressed. Tel/Fax: +84-42-383-7733, E-mail: asakura{at}cc.tuat.ac.jp

Silk has a long history of use in medicine as sutures. To address the requirements of a mechanically robust and biocompatible material, basic research to clarify the role of repeated sequences in silk fibroin in its structures and properties seems important as well as the development of a processing technique suitable for the preparation of fibers with excellent mechanical properties. In this study, three silk-like protein analogs were constructed from two regions selected from among the crystalline region of Bombyx mori silk fibroin, (GAGSGA)2, the crystalline region of Samia cynthia ricini silk fibroin, (Ala)12, the crystalline region of spider dragline silk fibroin, (Ala)6, and the Gly-rich region of spider silk fibroin, (GGA)4. The silk-like protein analog constructed from the crystalline regions of the spider dragline silk and B. mori silk fibroins, (A6SCS)8, that constructed from the crystalline regions of the S. c.ricini and B. mori silk fibroins, (A12SGS)4, that constructed from and the crystalline region of S. c.ricini silk fibroin and the glycine-rich region of spider dragline silk fibroin, (A12SGS)4,were expressed their molecular weights being about 36.0 kDa, 17.0 kDa and 17.5 kDa, respectively in E. coli by means of genetic engineering technologies. (A12SCS)4 and (A12SGS)4 undergo a structural transition from {alpha}-helix to ß-sheet on a change in the solvent treatment from trifluoroacetic acid (TFA) to formic acid (FA). However, (A6SCS)8 takes on the ß-sheet structure predominantly on TFA treatment and FA treatment. Structural analysis was performed on model peptides selected from spider dragline and S. c.ricini silks by means of 13C CP/MAS NMR.


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