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Journal of Biochemistry 2005 138(1):21-25; doi:10.1093/jb/mvi103
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© 2005 The Japanese Biochemical Society

Regular Paper

Probing of Specific Binding of Synthetic Sulfonylurea with the Insulinoma Cell Line MIN6

Keun-Hong Park1 and Toshihiro Akaike2,*

1 College of Medicine, Pochon CHA University, Cell and Gene Therapy Research Institute 605, Yeoksam 1-dong, Kangnam-gu, Seoul 135-081, Korea; and 2 Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501

* To whom correspondence should be addressed. Fax: +81-45-924-5815, E-mail:takaike{at}bio.titech.ac.jp

To overcome the limitation of conventional sulfonylurea (SU) for investigation of biological mechanisms related to KATP channels, a hypoglycemic sulfonylurea (SU) was conjugated with a non-reducing glucose bearing polystyrene (PS) derivative to provide enhanced interaction with an insulinoma cell line (MIN6). The specific interaction between the SU (K+ channel closer)-conjugated copolymer and MIN6 cells was confirmed by confocal laser microscopic images using rhodamine B isothiocyanate (RITC)–labeled SU-conjugated polymer, which revealed the specific interaction between SU-conjugated polymer and MIN6 cells. Moreover, the location of labeled polymer and the site of Ca2+ ion mobilization obtained from the same MIN6 cells were identical. Based on the specificity and insulinotropic activity, the SU-conjugated polymer is expected to be useful tool for the study of biological mechanisms of KATP channels.


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