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Journal of Biochemistry 2005 138(2):145-150; doi:10.1093/jb/mvi109
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© 2005 The Japanese Biochemical Society

Regular Paper

Molecular Character of the Recombinant Antitumor Lectin from the Edible Mushroom Agrocybe aegerita

Na Yang1,3,*, Xin Tong2,*, Ye Xiang1, Ying Zhang1, Yi Liang2, Hui Sun2,{dagger} and Da-Cheng Wang1,{dagger}

1 Center for Structural and Molecular Biology, Institute of Biophysics, Chinese Academy of Science, Beijing, 100101, People’s Republic of China; 2 State Key Laboratory of Virology, College of Life Science, Wuhan University, Wuhan, 430072, People’s Republic of China; and 3 Graduate School of Chinese Academy of Sciences, Beijing 100039, People’s Republic of China

{dagger} To whom correspondence should be addressed. Da-Cheng Wang, Tel: +86-10-64888547; Fax: +86-10-64888560, E-mail: dcwang{at}sun5.ibp.ac.cn; Hui Sun, Tel: +86-27-68754401, Fax: +86-27-68754401, E-mail: sunhui{at}whu.edu.cn

The lectin from Agrocybe aegerita (AAL) has been found to possess potent tumor-suppressing function and tumor cell apoptosis-inducing activity. In this paper, we report the full sequence, the active expression of the gene encoding AAL at a high level and bioassay of the binding property with lactose, apoptosis-inducing activity and DNase activity of recombinant AAL (rAAL). The results reveal that AAL is a member of the galectin family and the dimeric form is the active unit for the functional performance. The rAAL showed comparable tumor cell apoptosis-inducing activity with the wild AAL but no DNase activity at all. The molecular characters revealed by this study are significant for the in-depth investigation of the functional mechanism of this interesting protein.

* Both authors contributed equally to this work.


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