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Journal of Biochemistry 2006 139(1):51-58; doi:10.1093/jb/mvj006
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© 2006 The Japanese Biochemical Society.

Regular Paper

Drosophila Damaged DNA Binding Protein 1 Contributes to Genome Stability in Somatic Cells

Kaori Shimanouchi1, Kei-ichi Takata1,2, Masamitsu Yamaguchi3, Shizuka Murakami1, Gen Ishikawa1, Ryo Takeuchi1, Yoshihiro Kanai1, Tatsushi Ruike1, Ryoichi Nakamura1, Yoko Abe1 and Kengo Sakaguchi1,*

1 Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510; 2 University of Pittsburgh Cancer Institute, Hillman Cancer Center, Research Pavilion, 5117 Centre Avenue, Suite 2.6, Pittsburgh, PA 15213, USA; and 3 Department of Applied Biology, Faculty of Textile Sciences, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585

* To whom correspondence should be addressed. Phone: +81-471-24-1501 (Ext. 3409), Fax: +81-471-23-9767, E-mail: kengo{at}rs.noda.tus.ac.jp

The damaged DNA-binding protein (DDB) complex consists of a heterodimer of p127 (DDB1) and p48 (DDB2) subunits and is believed to have a role in nucleotide excision repair (NER). We used the GAL4-UAS targeted expression system to knock down DDB1 in wing imaginal discs of Drosophila. The knock-down was achieved in transgenic flies using over-expression of inverted repeat RNA of the D-DDB1 gene [UAS-D-DDB1(650)-dsRNA]. As a consequence of RNA interference (RNAi), the fly had a shrunken wing phenotype. The wing spot test showed induced genome instability in transgenic flies with RNAi knock-down of D-DDB1 in wing imaginal discs. When Drosophila larvae with RNAi knock-down of D-DDB1 in wing imaginal discs were treated with the chemical mutagen methyl methanesulfonate (MMS), the frequency of flies with a severely shrunken wing phenotype increased compared to non-treated transgenic flies. These results suggested that DDB1 plays a role in the response to DNA damaged with MMS and in genome stability in Drosophila somatic cells.


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C. A. Lovejoy, K. Lock, A. Yenamandra, and D. Cortez
DDB1 Maintains Genome Integrity through Regulation of Cdt1
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