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Journal of Biochemistry 2006 139(3):323-327; doi:10.1093/jb/mvj070
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© 2006 The Japanese Biochemical Society.

Rapid Communication

Application of Lectin Microarray to Crude Samples: Differential Glycan Profiling of Lec Mutants

Youji Ebe1,2, Atsushi Kuno2, Noboru Uchiyama2, Shiori Koseki-Kuno2, Masao Yamada1, Takashi Sato2, Hisashi Narimatsu2 and Jun Hirabayashi2,*

1 Nano/Bio Science Research Lab., Yokohama Technical Center, Moritex Corporation, 1-3-3 Azamino-Minami, Aoba-ku, Yokohama, Kanagawa 225-0012; and 2 Research Center for Glycoscience (RCG), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1, Umezono, Tsukuba, Ibaraki 305-8568

* To whom correspondence should be addressed. Phone: +81-29-861-3124, Fax: +81-29-861-3125, E-mail: jun-hirabayashi{at}aist.go.jp

We recently developed a novel system for lectin microarray based on the evanescent-field fluorescence-detection principle, by which even weak lectin-oligosaccharide interactions are detectable without a washing procedure. For its practical application, cell glycan analysis was performed for Chinese hamster ovary (CHO) cells and their glycan profile was compared with those of their glycosylation-defective Lec mutants. Each of the cell surface extracts gave a significantly different profile from that of the parental CHO cells in a manner reflecting denoted biosynthetic features. Hence, the developed lectin microarray system is considered to be fully applicable for differential glycan profiling of crude samples.


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