Skip Navigation

Journal of Biochemistry 2006 139(4):697-703; doi:10.1093/jb/mvj086
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Nagino, K.
Right arrow Articles by Tsujimoto, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagino, K.
Right arrow Articles by Tsujimoto, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2006 The Japanese Biochemical Society.

Regular Paper

Ultrasensitive DNA Chip: Gene Expression Profile Analysis without RNA Amplification

Kunihisa Nagino*,{dagger}, Osamu Nomura{dagger}, Yuki Takii{dagger}, Akira Myomoto{dagger}, Makiko Ichikawa{dagger}, Fumio Nakamura{dagger}, Masashi Higasa{dagger}, Hideo Akiyama{dagger}, Hitoshi Nobumasa{dagger}, Satoshi Shiojima{ddagger} and Gozoh Tsujimoto{ddagger}

New Frontiers Research Laboratories, Toray Industries, Inc., Kanagawa 248-8555; and Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501

* To whom correspondence should be addressed. E-mail: Kunihisa_nagino{at}nts.toray.co.jp

We have developed a new DNA chip whose substrate has a unique minute columnar array structure made of plastic. The DNA chip exhibits ultrahigh sensitivity, up to 100-fold higher than that of reference DNA chips, which makes it possible to monitor gene expression profiles even with very small amounts of RNA (0.1–0.01 µg of total RNA) without amplification. Differential expression ratios obtained with the new DNA chip were validated against those obtained with quantitative real-time PCR assays. This novel microarray technology would be a powerful tool for monitoring gene expression profiles, especially for clinical diagnosis.

{dagger} Present address: Toray Industries, Inc.

{ddagger} Present address: Kyoto University.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
Z. Zhao, R. Peytavi, G. A. Diaz-Quijada, F. J. Picard, A. Huletsky, E. Leblanc, J. Frenette, G. Boivin, T. Veres, M. M. Dumoulin, et al.
Plastic Polymers for Efficient DNA Microarray Hybridization: Application to Microbiological Diagnostics
J. Clin. Microbiol., November 1, 2008; 46(11): 3752 - 3758.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.