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Journal of Biochemistry 2006 139(5):837-846; doi:10.1093/jb/mvj095
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© 2006 The Japanese Biochemical Society.

Regular Paper

An Efficient RNA Aptamer against Human Influenza B Virus Hemagglutinin

Subash C.B. Gopinath, Yuriko Sakamaki, Kazunori Kawasaki and Penmetcha K.R. Kumar*

Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1 Higashi, Tsukuba, Ibaraki 305-8566

* To whom correspondence should be addressed. Tel: +81-298-61-6085, Fax: +81-298-61-6095, E-mail: pkr-kumar{at}aist.go.jp

Aptamers are known for their higher discriminating ability between closely related molecules and their requirement for only a small region for binding, as compared to an antibody. In the present studies, we have isolated a specific RNA aptamer against the influenza virus B/Johannesburg/05/1999 by an in vitro selection procedure. The aptamer bound efficiently to the HA of influenza B and required 5 mM MgCl2 ion for its recognition. The aptamer not only distinguished HA derived from the influenza A virus, but also inhibited HA-mediated membrane fusion.


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