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Journal of Biochemistry Advance Access originally published online on July 14, 2006
Journal of Biochemistry 2006 140(2):255-265; doi:10.1093/jb/mvj153
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© 2006 The Japanese Biochemical Society.

Regular Paper

Establishment and Functional Characterization of Novel Natural Killer Cell Lines Derived from a Temperature-Sensitive SV40 Large T Antigen Transgenic Mouse

Satoru Iizuka1, Tomonori Kaifu1, Akira Nakamura1, Masuo Obinata2 and Toshiyuki Takai1,*

1 Department of Experimental Immunology and the CREST Program of the Japan Science and Technology Corporation (JST) and 2 Department of Cell Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575

* To whom correspondence should be addressed. Department of Experimental Immunology, Institute of Development, Aging and Cancer, Tohoku University, Seiryo 4-1, Sendai 980-8575. Phone: +81 22 717 8501, Fax: +81 22 717 8505, E-mail: tostakai{at}idac.tohoku.ac.jp

Natural killer (NK) cells belong to an important lymphocyte population that eliminates transformed cells and invading pathogens without any prior sensitization. NK cells possess not only natural killing activity against non-self and altered-self cells but also exhibit cytokine production and antibody-dependent cell-mediated cytotoxicity (ADCC). Despite their important roles in the innate immune system, little is known about the details of NK cell biology. In spite of that several murine NK cell clones have been established, studies have mainly focused on their natural killing activity but not their cytokine production or ADCC. In this study, we established and characterized eight novel, immortalized murine NK cell clones derived from a temperature-sensitive SV40 large-T antigen transgenic mouse. These NK cell lines continuously proliferated for more than 30 months in a culture medium supplemented with interleukin 2. All cell lines contained azurophilic granules in the cytoplasm, and a few clones retained the NK cell functions, such as natural killing activity, cytokine production, and ADCC. In addition, one clone could serve as a host for transient as well as stable gene transfection. Taken together, these findings indicate that the cell lines could constitute useful tools for detailed analysis of murine NK cell biology.


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