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Journal of Biochemistry Advance Access originally published online on July 14, 2006
Journal of Biochemistry 2006 140(2):267-273; doi:10.1093/jb/mvj151
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© 2006 The Japanese Biochemical Society.

Regular Paper

Platelet Derived Growth Factor Receptor Alpha Is Essential for Establishing a Microenvironment That Supports Definitive Erythropoiesis

Wen-Ling Li1, Yoshihiro Yamada2, Masaya Ueno2, Satomi Nishikawa3, Shin-Ichi Nishikawa3 and Nobuyuki Takakura1,2,*

1 Department of Stem Cell Biology, Cancer Research Institute of Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-0934; 2 Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871; and 3 Laboratory for Stem Cell Biology, RIKEN Center for Developmental Biology, 2-2-3 Minatojima Minami-machi, Kobe, Hyogo 650-0047

* To whom correspondence should be addressed at: Department of Signal Transduction, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871. Tel: +81-6-6879-8316, Fax: +81-6-6879-8314, E-mail: ntakaku{at}biken.osaka-u.ac.jp

The hematopoietic system undergoes a qualitative change during the embryogenesis of most vertebrates. It is designated as the shift of primitive to definitive hematopoiesis and suitable microenvironment must be established to support this shift. While studying the role of platelet derived growth factor receptor {alpha} (PDGFR {alpha}) in embryonic hematopoiesis, we found that it was expressed in a stromal cell component of liver, a major site of this shift, but not in the yolk sac, the site of primitive hematopoiesis. Thus, we considered that development of PDGFR{alpha} positive stromal cells is an essential requirement for this shift. Without PDFGR{alpha} positive cell component, erythropoiesis was suppressed in the culture of fetal liver. Moreover, injection of an antagonistic anti-PDGFR{alpha} monoclonal antibody during embryogenesis suppressed the production of definitive erythrocytes. These indicated that PDGF exerts its effect on a subset of stromal components to prepare a microenvironment that can support the definitive erythropoiesis.


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