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Journal of Biochemistry Advance Access originally published online on February 13, 2007
Journal of Biochemistry 2007 141(4):479-488; doi:10.1093/jb/mvm048
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© 2007 The Japanese Biochemical Society.

Identification of Novel Members of the Xenopus Ca2+-dependent Lectin Family and Analysis of Their Gene Expression During Tail Regeneration and Development

Tomoko Ishino1,*, Takekazu Kunieda2,*, Shunji Natori3, Kazuhisa Sekimizu1 and Takeo Kubo2,{dagger}

1Graduate School of Pharmaceutical Sciences, University of Tokyo; 2Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033; and 3National Institute of Agrobiological Sciences, an Independent Administrative Institution, 1-2 Ohwashi, Tsukuba, Ibaraki 305-8634, Japan

{dagger}To whom correspondence should be addressed. Tel. +81-3-5841-4446, Fax: +81-3-5841-4447, E-mail: stkubo{at}biol.s.u-tokyo.ac.jp

Received October 19, 2006; Accepted January 20, 2007


  Abstract

We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987–994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole, we isolated a 35-kDa Ca2+-dependent lectin (XCL-1) from adult Xenopus serum and cloned its cDNA. Although XCL-1 gene expression was not induced in the regenerating tails, we isolated a cDNA for an XCL-1-related protein (XCL-2) by reverse transcription-polymerase chain reaction. In contrast to the XCL-1 gene, XCL-2 gene expression was significantly increased in the regenerating tails, suggesting its role in tail regeneration. Although both XCL-1 and XCL-2 belong to a recently identified Xenopus lectin family (X-lectins), XCL-1 and XCL-2 exhibit distinct developmental gene expression from two other known X-lectin members, both of which are expressed principally in the embryonic stage, whereas the XCL-1 and XCL-2 genes are predominantly expressed in the adult and middle/late tadpole stages, respectively, suggesting multiple functions of X-lectin family members. Thus, the presence of multifunctional Ca2+-dependent lectin family and the induction of the member gene in regenerating organs are conserved among insects and vertebrates.

Key Words: development, gene expression, regeneration, serum lectin, Xenopus laevis

Abbreviations: EF-1{alpha}, elongation factor 1{alpha}; PCR, polymerase chain reaction; RACE, rapid amplification of cDNA ends; RT, reverse transcription

*The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors.


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