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Journal of Biochemistry Advance Access originally published online on July 23, 2007
Journal of Biochemistry 2007 142(3):351-355; doi:10.1093/jb/mvm141
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© 2007 The Japanese Biochemical Society.

Involvement of Sphingosine 1-Phosphate, a Platelet-Derived Bioactive Lipid, in Contraction of Mesangium Cells

Makoto Osada1, Yutaka Yatomi2,*, Tsukasa Ohmori1, Shinya Aoki2, Shigemi Hosogaya1 and Yukio Ozaki1

1Department of Clinical and Laboratory Medicine, Faculty of Medicine, University of Yamanashi, Yamanashi; and 2Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

*To whom correspondence should be addressed. Tel: +81-3-5800-8721, Fax: +81-3-5689-0495, E-mail: yatoyuta-tky{at}umin.ac.jp

Received March 30, 2007; Accepted June 19, 2007


  Abstract

Platelet-derived mediators may play an important role in the development of renal diseases through interaction with glomerular mesangial cells (MCs), and we, in this study, examined the effect of sphingosine 1-phosphate (Sph-1-P), a bioactive lipid released from activated platelets, on the contraction of MCs. Sph-1-P was found to induce MC contraction through mediation of Rho kinase both in cell shape change and collagen gel contraction assays. The specific antagonist of the Sph-1-P receptor S1P2 inhibited the response. Similar results were obtained when the supernatant from activated platelet suspensions were used instead of Sph-1-P. Our findings suggest that platelet-derived Sph-1-P may be involved in MC contraction via S1P2 and that regulation of this receptor might be useful therapeutically.

Key Words: contraction, mesangial cell, platelet, Rho kinase, S1P2, sphingosine 1-phosphate

Abbreviations: MC, mesangial cell; Sph-1-P, sphingosine 1-phosphate


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