Skip Navigation

Journal of Biochemistry 2007 142(5):561-570; doi:10.1093/jb/mvm171
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Fan, J.
Right arrow Articles by Wang, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fan, J.
Right arrow Articles by Wang, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2007 The Japanese Biochemical Society

Molecular Characterization of Plantain Class I Chitinase Gene and its Expression in Response to Infection by Gloeosporium musarum Cke and Massee and other Abiotic Stimuli*

Jianming Fan{dagger}, Hongbin Wang{dagger}, Dongru Feng, Bin Liu, Haiyan Liu and Jinfa Wang{ddagger}

The State Key Laboratory of Biocontrol and The Key Laboratory of Gene Engineering of Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China

{ddagger}To whom correspondence should be addressed. Tel: 86-20-84039179, Fax: 86-20-84039179, E-mail: xibao{at}mail.sysu.edu.cn

Received May 8, 2006; Accepted July 16, 2007


  Abstract

We have cloned a chitinase cDNA (MpChi-1) from plantain (Musa paradisiacal L) using rapid amplification of cDNA ends (RACE) according to a sequence fragment which we had cloned using the suppression subtractive hybridization (SSH) technique. The MpChi-1 encodes a protein of 326 amino acids and belongs to acidic chitinase class Ib subfamily. MpChi-1 shares high identity with rice endochitinase (XP_468714) and different each other only at three residues. Homology modelling indicated these three substitutions would not change the configuration of the activity site of the enzyme. We have expressed recombinant MpChi-1 and purified by ammonium sulphate precipitation and preparative reversed phase HPLC. The recombinant protein could hydrolyse chitin and inhibit the growth of the Gloeosporium musarum Cke and Massee in vitro. Northern blot revealed that the MpChi-1 transcripts rapidly after inoculation with G. musarum and maximum mRNA accumulation reached at 48 h. Jasmonic acid (JA) and salicylic acid (SA) could induce MpChi-1 expression, while mechanical wounding, silver nitrate and osmotic stress stimulated only a slight accumulation of MpChi-1 transcripts. Abscisic acid (ABA) could induce MpChi-1 transcript. These results suggest the MpChi-1 plays important role in the events of the hypersensitive reaction (HR).

Key Words: chitinases gene, hypersensitive reaction, induction, plantain, recombinant protein

Abbreviations: ABA, Abscisic acid; HR, hypersensitive reaction; JA, Jasmonic acid; MpChi-1, Musa paradisiacal chitinase; PR, pathogenesis-related; SA, salicylic acid; SSH, suppression subtractive hybridization

*Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number(s) AY997529 and DN796037.

{dagger}These two authors contributed equally to this work.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.