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Journal of Biochemistry Advance Access originally published online on November 4, 2007
Journal of Biochemistry 2008 143(2):179-186; doi:10.1093/jb/mvm209
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© 2007 The Japanese Biochemical Society.

Effect of Low Doses of Red Wine and Pure Resveratrol on Circulating Endothelial Progenitor Cells

Maria Luisa Balestrieri1,*, Concetta Schiano2, Francesca Felice1, Amelia Casamassimi2, Antonio Balestrieri1, Lara Milone1, Luigi Servillo1 and Claudio Napoli2,3

1Department of Biochemistry and Biophysics; 2Department of General Pathology, Division of Clinical Pathology; and 3Excellence Research Center of Cardiovascular Diseases, II University of Naples, Naples 80138, Italy

*To whom correspondence should be addressed. Tel: +39-081-5667587, Fax: +39-081-5665863, E-mail: marialuisa.balestrieri{at}unina2.it

Received September 14, 2007; Accepted October 10, 2007


  Abstract

Circulating endothelial progenitor cells (EPCs) play a significant role in neovascularization of ischaemic tissues and in re-endothelization of injured blood vessels. Identification of compounds able to enhance EPC levels and improve their functional activity, noticeably compromised by risk factors for coronary heart disease, is of clinical interest. This study evaluates the effects of red wine on EPCs. After being isolated from total peripheral blood mononuclear cells, EPC phenotype was confirmed by the presence of double positive cells for DiLDL uptake and lectin binding and by expression of CD34, CD133 and VE-cadherin cell surface markers. Long-term culture in the presence of red wine (1 µl/ml), containing resveratrol (Resv) at physiological concentration (nM), determined a time-dependent amelioration of cell number (P < 0.05). The presence of red wine prevented the TNF-{alpha}-induced reduction of EPC number (P < 0.05) and this effect was accompanied by reduced p38-phosphorylation expression levels (P < 0.05) and increased NOx levels (P < 0.05) Indeed, pure Resv alone significantly improved the TNF-{alpha} reduced EPC number (P < 0.05). This evidence indicates novel beneficial effects of red wine and Resv in the positive modulation of EPCs levels.

Key Words: antioxidants, endothelial progenitor cells, red wine, resveratrol

Abbreviations: CHD, coronary heart disease; DilLDL, 1,10-dioctadecyl-3,3,30,30-tetramethylindocarbocyanina-labelled acetylated LDL; EC, endothelial cells; EPCs, Endothelial progenitor cells; LDL, low density lipoprotein; LDLR–/–, hypercholesterolaemic LDL receptor-deficient; MAP, kinase, mitogen activating protein kinase; NO, nitric oxide; eNOS, endothelial nitric oxide synthase; PBMCs, peripheral blood mononuclear cells; PE, phycoerythrin; TNF-{alpha}, tumour necrosis factor-alpha; RW, red wine; Resv, resveratrol; VE-cad, VE-cadherin; VEGF, vascular endothelial growth factor


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