Journal of Biochemistry Advance Access originally published online on January 23, 2008
Journal of Biochemistry 2008 143(5):581-592; doi:10.1093/jb/mvn006
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© 2008 The Japanese Biochemical Society.
Matrix Metalloproteinase-9 Associated with Heparan Sulphate Chains of GPI-Anchored Cell Surface Proteoglycans Mediates Motility of Murine Colon Adenocarcinoma Cells
1Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Kamigamo Motoyama, Kitaku, Kyoto 603-8555; 2Clinical Research Center, National Hospital Organization Nagoya Medical Center, 4-1-1 Sannomaru, Nakaku, Nagoya 460-0001; 3Department of Medical Technology, Nagoya University School of Health Sciences, 1-1-20 Daikohigashi, Higashiku, Nagoya 461-8673; and 4Department of Oncological Pathology, Aichi Cancer Center Research Institute, 1-1 Kanokoden, Chikusaku, Nagoya 464-0021, Japan
*To whom correspondence should be addressed. Tel: +81 52 951 1111, Fax: +81 52 951 9075, E-mail: ogurik{at}nnh.hosp.go.jp
Received September 25, 2007; Accepted January 4, 2008
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Abstract |
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Using murine colon adenocarcinoma-derived clones with different metastatic potentials, the cellular localization of matrix metalloporteinase-9 (MMP-9) and its role in the cell motility were examined. Highly metastatic LuM1 clone aggressively invaded into adjacent tissue in vivo, but low metastatic NM11 clone did not. As compared with the NM11 clone, the LuM1 clone expressed and secreted a remarkably large amount of MMP-9, and exhibited higher abilities of cell migration and invasion in vitro, which were suppressed by MMP-2/MMP-9 inhibitor IV. MMP-9, exhibiting high affinity to heparin, was demonstrated to be condensed on tips of cellular podia. Treatment of the cells with heparitinase-I or heparin resulted in release of MMP-9 from the cell surface, which caused concomitant suppression of their motility to a similar level to that with the MMP inhibitor. Immunoprecipitation of a LuM1 cell lysate with an anti-MMP-9 antibody resulted in co-precipitation of phosphatidylinositol-specific phospholipase C-susceptible heparan sulphate proteoglycans having 66 and 64 kDa core proteins. Taken together, the present results demonstrate that secreted MMP-9 associates with glypican-like proteoglycans through their heparan sulphate chains, and plays a crucial role in cell motility of LuM1 cells.
Key Words: cell motility, cell surface heparan sulphate, glypican, invasion, matrix metalloproteinase-9
Abbreviations: APMA, p-aminophenylmercuric acetate; DAPI, 4,6-diamidino-2-phenylindole; FCS, foetal calf serum; FITC, fluorescein isothiocyanate; GPI, glycosylphosphatidylinositol; MMP, matrix metalloproteinase; MT, membrane-type; PI-PLC, phosphatidylinositol-specific phospholipase C; RT, reverse transcriptase; TBS, Tris-buffered saline; TIMP, tissue inhibitor of metalloproteinases
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