Skip Navigation


Journal of Biochemistry Advance Access originally published online on January 23, 2008
Journal of Biochemistry 2008 143(5):611-616; doi:10.1093/jb/mvn008
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
143/5/611    most recent
mvn008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Nagayasu, Y.
Right arrow Articles by Yokoyama, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagayasu, Y.
Right arrow Articles by Yokoyama, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 2008 The Japanese Biochemical Society.

Reactivity of Astrocytes to Fibroblast Growth Factor-1 for Biogenesis of Apolipoprotein E-High Density Lipoprotein is Down-regulated by Long-time Secondary Culture

Yuko Nagayasu, Jin-ichi Ito, Tomo Nishida and Shinji Yokoyama*

Biochemistry, Nagoya City University Graduate School of Medical Sciences, Kawasumi 1, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan

*To whom correspondence should be addressed. Tel: +81 52 853 8139, Fax: +81 52 841 3480, E-mail: syokoyam{at}med.nagoya-cu.ac.jp

Received October 30, 2007; Accepted January 15, 2008


  Abstract

We previously showed that astrocytes produce and release fibroblast growth fator-1 (FGF-1) upon 1-month primary and 1-week secondary culture (M/W cells) and stimulate themselves by an autocrine manner to produce apoE-high-density lipoproteins (HDL), closely associated with their generation of apoE-HDL in brain injury. Astrocytes prepared by 1-week primary and 1-month secondary culture (W/M cells), however, expressed FGF-1 as much as M/W cells but produce apoE-HDL much less. The W/M cells conditioned medium in fact contained FGF-1 activity to stimulate astrocytes prepared by 1-week primary and 1-week-secondary culture (W/W cells). FGF-1 did not stimulate W/M cells for apoE-HDL biogenesis while it stimulated W/W cells. Phosphorylation of Akt, ERK and MEK were induced by FGF-1 in W/W cells but not in W/M cells. Finally, fibroblast growth factor receptor-1 in the membrane decreased in W/M cells in comparison to W/W cells. Interestingly, the reactivity of astrocytes to FGF-1 was recovered when W/M cells were transferred to the tertiary culture of 1 week. We concluded that astrocytes decrease their reactivity to FGF-1 for apoE-HDL biogenesis in certain conditions. The findings indicate astrocyte FGF-1 enhances biogenesis of apoE-HDL also by a paracrine mechanism.

Key Words: astrocytes, apolipoprotein E, high-density lipoprotein, cholesterol, fibroblast growth factor-1

Abbreviations: apoE, apolipoprotein E; HDL, high-density lipoprotein; FGF-1, fibroblast growth factor 1; DPBS, Dulbecco's phosphate-buffered saline; FCS, fetal calf serum; TLC, thin layer chromatography; BSA, bovine serum albumin; FGFR-1, fibroblast growth factor receptor 1


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.