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Journal of Biochemistry Advance Access originally published online on September 3, 2008
Journal of Biochemistry 2008 144(4):415-418; doi:10.1093/jb/mvn107
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© 2008 The Japanese Biochemical Society.

Rapid Communication

Isolation of Small RNAs using Biotinylated PNAs

Takashi Ohtsuki*, Takeshi Fujimoto, Maya Kamimukai, Chisato Kumano, Mizuki Kitamatsu and Masahiko Sisido

Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan

*To whom correspondence should be addressed. Tel: +81-86-251-8220, Fax: +81-86-251-8220, E-mail: ohtsuk{at}cc.okayama-u.ac.jp

Received August 2, 2008; Accepted August 28, 2008


  Abstract

In this study, an RNA isolation method was developed using a biotinylated peptide nucleic acid (PNA) that is complementary to the target RNA. Using the biotinylated PNA method, we successfully isolated several RNAs from Escherichia coli and from human total RNA in pure form. Damage to the RNA appears to be negligible by this method because the method is rapid and does not require a high temperature treatment to facilitate RNA–PNA binding.

Key Words: non-coding RNA, peptide nucleic acid, ribosomal RNA, RNA isolation, transfer RNA

Abbreviations: EDTA, ethylenediaminetetraacetic acid; PNA, peptide nucleic acid


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