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Journal of Biochemistry Advance Access originally published online on September 6, 2008
Journal of Biochemistry 2008 144(5):625-633; doi:10.1093/jb/mvn109
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© 2008 The Japanese Biochemical Society

Intercellular Accumulation of Type V Collagen Fibrils in Accordance with Cell Aggregation

Takanori Kihara1, Yasutada Imamura2,*, Yukitoshi Takemura3, Kazunori Mizuno4, Eijiro Adachi5 and Toshihiko Hayashi6

1Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, Tokyo 113-8656; 2Department of Applied Chemistry, Kogakuin University, Tokyo 192-0015; 3Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan; 4Shriners Hospital for Children, Portland Research Center, OR 97239, USA; 5Department of Molecular Morphology, Graduate School of Medical Sciences, Kitasato University, Kanagawa 228-8555; and 6Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Chiba 290-0193, Japan

*To whom correspondence should be addressed. Tel: +81-42-628-4862, Fax: +81-42-628-4862, E-mail: bt40522{at}ns.kogakuin.ac.jp

Received August 4, 2008; Accepted August 18, 2008


  Abstract

We reported previously that human fibroblasts form clumps when cultured on a dish coated with reconstituted type V collagen fibrils. Essentially all the type V collagen fibrils, initially coated on the dish, were recovered in the cell clumps that had eventually formed during the culture. We interpreted that type V collagen fibrils adhere to cells more strongly than to the dish and are detached by cell movements. In this study, type V collagen was suspended with fibroblasts to examine the fate of the type V collagen fibrils and to determine whether the fibrils affect the behaviour of the cells directly adherent to the dish. The added type V collagen accumulated in the intercellular space concomitantly with the local aggregation of fibroblasts. scanning electron microscope examination indicated that type V collagen fibrils were found in the vicinity of cells in cultures without ascorbic acid where essentially no collagen secretion takes place. These results indicate that type V collagen forms fibrils and the fibrils are accumulated in the intercellular spaces. The accumulated type V collagen fibrils work as a cementing material for cell clump formation. This phenomenon is discussed in relation to the possible involvement of type V collagen fibrils in tissue organization.

Key Words: accumulation of collagen fibrils, cell cementing, cell–collagen interaction, clump formation, type V collagen fibrils

Abbreviations: PBS, calcium-free magnesium-free phosphate buffered saline; FITC, fluorescein isothiocyanate; SEM, scanning electron microscope


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