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Journal of Biochemistry Advance Access originally published online on October 3, 2008
Journal of Biochemistry 2008 144(6):717-724; doi:10.1093/jb/mvn126
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© The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Effects of Dimethyl Sulphoxide and Dexamethasone on mRNA Expression of Myogenesis- and Muscle Proteolytic System-related Genes in Mouse Myoblastic C2C12 Cells

Masuhiro Nishimura1,*, Mayumi Mikura1,2, Katsuya Hirasaka2, Yuushi Okumura2, Takeshi Nikawa2, Yuichi Kawano1, Mitsuo Nakayama1 and Muneharu Ikeda1

1New Product and Business Development, Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Naruto, Tokushima 772-8601; and 2Department of Nutritional Physiology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan

*To whom correspondence should be addressed. Tel: +81-88-685-1151 (ext 334), Fax: +81-88-686-8176, E-mail: nisimums{at}otsukakj.co.jp

Received May 7, 2008; Accepted September 13, 2008


  Abstract

We examined the time course of mRNA expression of myogenic cell differentiation- and muscle proteolytic system-related genes in cultures of C2C12 cells during differentiation from myoblasts to myotubes. Furthermore, we treated C2C12 myotubes with dimethyl sulphoxide (DMSO) and dexamethasone (Dex), and examined changes in these mRNA levels. Myogenin (Myog), Atrogin1, forkhead box O1 (Foxo1) and Capn1 mRNA levels increased in C2C12 cells differentiating from myoblasts to myotubes, whereas Myf5 mRNA levels decreased. Although genes such as MRF4, Foxo3a, UbB, Capn1 and MuRF1 mRNAs in the myotubes were affected by DMSO exposure, mRNA levels of other genes were not markedly affected by exposure to 0.02% or 0.5% DMSO. Myf5, MRF4, Atrogin1, Foxo3 and MuRF1 mRNA levels were elevated by Dex at all time points, Cbl and Capn1 mRNA levels were significantly elevated by Dex at 8 h, and Myog mRNA levels were significantly elevated by Dex at 24 h. However, CtsH mRNA levels decreased significantly with Dex at 24 h. This study provides a useful database of gene profiles that are differentially expressed throughout myogenic cell differentiation and the muscle proteolytic system.

Key Words: C2C12 myotubes, dexamethasone, dimethyl sulphoxide, muscle proteolytic system-related genes, myogenesis-related genes

Abbreviations: Capn1, calpain 1; Capn2, calpain 2; Cbl, Casitas B-lineage lymphoma; CtsB, cathepsin B; CtsH, cathepsin H; Dex, dexamethasone; DMSO, dimethyl sulphoxide; Foxo1, forkhead box O1; Foxo3a, forkhead box O3a; HPRT1, hypoxanthine phosphoribosyltransferase 1; MRF, myogenic regulatory factor; MRF4, myogenic regulatory factor 4; MuRF1, muscle RING finger 1; Myf5, myogenic factor 5; Myog, myogenin; Myod1, myogenic differentiation 1; UbB, ubiquitin B; Ubr1, ubiquitin protein ligase E3 component n-recognin 1; Ubr2, Ubiquitin protein ligase E3 component n-recognin 2


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