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Journal of Biochemistry Advance Access originally published online on December 12, 2008
Journal of Biochemistry 2009 145(3):275-278; doi:10.1093/jb/mvn169
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© The Authors 2008. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Rapid Communication

Human Stem Cell Factor (SCF) is a Heparin-Binding Cytokine

Satoko Kishimoto1,2, Shingo Nakamura3, Hidemi Hattori1, Shin-ichiro Nakamura4, Fumie Oonuma1, Yasuhiro Kanatani1, Yoshihiro Tanaka1, Yasutaka Mori1, Yasuji Harada2, Masahiro Tagawa2 and Masayuki Ishihara1,*

1Research Institute, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513; 2Department of Surgery, Graduate School of Nippon Veterinary Medicine and Life Science University, 1-7-1, Kyonan-cho, Musashino-shi, Tokyo, 180-8602; 3Department of Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa; and 4Department of Plastic and Reconstructive Surgery, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan

*To whom correspondence should be addressed: Tel: +81-42-995-1601, Fax: +81-42-991-1611, E-mail: ishihara{at}ndmc.ac.jp

Received October 2, 2008; Accepted December 2, 2008


  Abstract

Binding affinities of chemically modified heparins for human stem cell factor (SCF) were examined using fragmin/protamine microparticles (F/P MPs) and an enzyme-linked immunosorbent assay (ELISA). The binding of SCF to F/P MP-coated plates was inhibited with high concentrations of heparin and fragmin, but not others. The binding of SCF was also inhibited with 0.55 M or higher concentrations of NaCl in the medium. These results suggested that a high content of all three sulfate groups in repeating disaccharide units is required for interaction with SCF. Furthermore, pre-immobilized SCF on F/P MP-coated plates significantly stimulated proliferation of a human erythroleukemia cell line.

Key Words: enzyme-linked immunosorbent assay (ELISA), fragmin/protamine microparticles (F/P MPs), heparin-binding cytokine, heparinoids, human stem cell factor (SCF)

Abbreviations: SCF, human stem cell factor; F/P MPs, fragmin (low-molecular-weight heparin)/protamine microparticles; ELISA, enzyme-linked immunosorbent assay; TF-1 cells, human erythroleukemia cell line; N-DS/N-Ac-H, N-desulfated, N-acetylated heparin; 2-O-DS-H, 2-O-desulfated heparin; 6-O-DS-H, 6-O-desulfated heparin


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