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Journal of Biochemistry Advance Access originally published online on January 20, 2009
Journal of Biochemistry 2009 145(4):425-427; doi:10.1093/jb/mvp011
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© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Rapid Communication

Peroxiredoxin III-deficiency Sensitizes Macrophages to Oxidative Stress

Lianqin Li1,2,*, Tomonori Kaifu3, Masuo Obinata2 and Toshiyuki Takai3

1Obstetrics and Gynecology Center, Tsinghua University Second Hospital, Beijing 100049 China; 2Department of Cell Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575; and 3Department of Experimental Immunology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575 Japan

*To whom correspondence should be addressed. Tel: +86 10 87002811, Fax: +86 10 88257362, E-mail: lilq2005{at}126.com

Received January 11, 2009; Accepted January 13, 2009


  Abstract

As a mitochondrial scavenger of reactive oxygen species (ROS), peroxiredoxin III (PrxIII) plays an important role in regulating intracellular ROS level. We previously found that PrxIII knockout (PrxIII–/–) mice were more sensitive than wild-type (PrxIII+/+) controls to intratracheal inoculation of lipopolysaccharide (LPS), but the precise mechanism remained to be obscure. In the present study, we detected the levels of ROS and tumour necrosis factor alpha (TNF-{alpha}) in mouse bone-marrow-derived macrophages. LPS stimulation induced transient increase of ROS production and augmentation of TNF-{alpha} accumulation in PrxIII–/– macrophages. In addition, we observed reduced viability and increased apoptosis in PrxIII–/– macrophages exposed to LPS. Our results provide direct evidence that PrxIII is necessary for macrophages to protect against LPS-induced oxidative stress.

Key Words: lipopolysaccharide, macrophage, mouse, peroxiredoxin III, reactive oxygen species

Abbreviations: DCFH-DA, 2',7'-dichlorofluorescin diacetate; DMEM, Dulbecco's modified Eagle's medium; ELISA, enzyme-linked immunosorbent assay; FBS, fetal bovine serum; LPS, lipopolysaccharide; PBS, phosphate-buffered saline; PrxIII, peroxiredoxin III; ROS, reactive oxygen species; TMB, 3,3',5,5'-tetramethylbenzidine; TNF-{alpha}, tumor necrosis factor alpha


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