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Journal of Biochemistry Advance Access originally published online on January 16, 2009
Journal of Biochemistry 2009 145(4):525-532; doi:10.1093/jb/mvp005
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© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Cloning and Expression of the MutM Gene from Obligate Anaerobic Bacterium Desulfovibrio vulgaris (Miyazaki F)*

Hideaki Sanada, Takeshi Nakanishi, Hideo Inoue and Masaya Kitamura{dagger}

Department of Applied and Bioapplied Chemistry, Graduate School of Engineering, Osaka City University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan

{dagger}To whom correspondence should be addressed. Tel: +81-6-6605-3091, Fax: +81-6-6605-2769, E-mail: kitamura{at}bioa.eng.osaka-cu.ac.jp

Received October 23, 2008; Accepted January 7, 2009


  Abstract

The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was composed of 336 amino acids. The amino-acid sequence deduced from the MutM gene was highly homologous with the MutM of other bacteria; however an additional insert consisted of 64 amino acids. An expression system for the MutM gene under the control of the T7 promoter was constructed in E. coli. From the kinetic analysis results, the purified His-tagged MutM showed 8-oxoguanine-DNA glycosylase activity comparable with that of MutM from E. coli. In this study, the amounts of mRNA and protein for MutM were scant in the D. vulgaris (Miyazaki F). MutM activity may be induced by oxidative stress. However, its induction may not be frequently generated because sulfate-reducing bacteria generally grow in anaerobic conditions. MutM might play a role in the protection against the mutagenicity of oxygen when oxygen stress exceeded the capacity of the defense systems against oxygen toxicity.

Key Words: MutM, obligate anaerobe, oxidative stress, recombinant, sulfate-reducing bacteria

Abbreviations: D., Desulfovibrio; ICP-AE, inductively coupled plasma-atomic emission; ORF, open reading frame; 8-oxoG, 8-oxo-7,8-dihydro-2'-deoxyguanine; SRB, sulfate-reducing bacteria


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