Skip Navigation


Journal of Biochemistry Advance Access originally published online on April 7, 2009
Journal of Biochemistry 2009 146(2):157-166; doi:10.1093/jb/mvp056
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
146/2/157    most recent
mvp056v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by Fei, X.
Right arrow Articles by Deng, X.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fei, X.
Right arrow Articles by Deng, X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

An Fe Deficiency Responsive Element with a Core Sequence of TGGCA Regulates the Expression of FEA1 in Chlamydomonas reinharditii

Xiaowen Fei1,2, Mats Eriksson3, Jinghao Yang1 and Xiaodong Deng1,*

1Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China; 2Department of Biochemistry, Hainan Medical College, Haikou 571101, China; and 3Umeå Plant Science Center, Umeå University, SE-901 87 Umeå, Sweden

*To whom correspondence should be addressed. Tel: +86-898-66960173; Fax: +86-898-66890978, E-mail: xiaodong9deng{at}hotmail.com

Received January 14, 2009; Accepted March 17, 2009


  Abstract

Iron is essential to the unicellular green alga Chlamydomonas, but the molecular mechanism for response to iron deficiency remains largely unknown. In previous studies, we have identified FOX1 and ATX1 FEREs (Fe deficiency-responsive elements) as important regulation components of iron response in this organism. Here we present another iron regulated gene FEA1, which promoter was analysed by using a 5'-and 3'-end deletion and a scanning mutagenesis assay. The results reveal that the co-existence of –273/–188 and –118/–49 regions from transcriptional start site of FEA1 were sufficient and necessary for Fe deficiency-induced expression. Further deletion analysis indicates both –273/–253 and –103/–85 regions are essential for inducible expression. The scanning mutagenesis analysis of these regions identifies two cis-acting elements: the FeaFeRE1 at –273/–259 (CTGCGGTGGCAAAGT) and FeaFeRE2 at –106/–85 (CCGCCGCNNNTGGCACCAGCCT). Sequence comparison of FeaFeRE1 and FeaFeRE2 reveals a core sequence of TGGCA, which had been found in our previously reported Fe-deficiency-inducible gene ATX1. Moreover, we show that the promoter region of several genes, including FRE1, IRT1, ISCA, ZRT1, ZRT5, NRAMP2 and COPT1, also contains this core sequence, suggesting that at least two classes FeRE elements exist in Clamydomonas, one in FEA1 and ATX1 and others the second in FOX1, FEA2, MTP4, NRAMP3 and RBOL1.

Key Words: Chlamydomonas reinharditii, FEA1, FeREs, iron, promoter

Abbreviations: FEA1, Fe assimilation 1; ATX1, antioxidant 1; FOX1, ferroxidase1; FER1, ferritin 1; Ars, arylsulfatase; FeREs, Fe deficiency-responsive elements


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.