Journal of Biochemistry Advance Access originally published online on April 7, 2009
Journal of Biochemistry 2009 146(2):185-191; doi:10.1093/jb/mvp058
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Distribution of Receptor Glycolipids for Lactobacilli in Murine Digestive Tract and Production of Antibodies Cross-reactive with them by Immunization of Rabbits with Lactobacilli*
1Department of Biochemistry, Faculty of Science and Technology, Kinki University, 3-4-1 Kowakae, Higashiosaka, Osaka 577-8502; and 2Animal Models of Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Asagi-Saito, Ibaraki, Osaka 567-0085, Japan
To whom correspondence should be addressed. Tel: +81 6 6721 2332, Fax: +81 6 6723 6721, E-mail: iwamori{at}life.kindai.ac.jp
Received February 12, 2009; Accepted March 23, 2009
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Abstract |
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In the digestive tract of mice (HR-1 strain), glycolipids belonging to the ganglio-series were revealed to be expressed in region-specific manners, i.e. FGA1 and FGM1 in the stomach, GA1 in the small intestine, and FGA1 and sulphatides in the cecum. The amount of GA1 as a receptor glycolipid for Lactobacilli was especially higher in the small intestine than in the other regions, it comprising 1.6–2.8 µg/mg dry weight. On immunization of rabbits with Lactobacillus johnsonii and Lactobacillus intestinalis, both of which are murine intestinal bacteria, antibodies toward bacterial glycolipids, i.e. Gal
1–2Glc1–3DG, and tri- and tetrahexaosyl DGs, were effectively generated and, in addition, they were found to cross-react with GA1 and GalCer, but not with structurally related glycolipids such as Lc4Cer, nLc4Cer and IV3Gal-nLc4Cer, indicating that GA1 is a preferable antigen for anti-lactobacillus antisera and suggesting the presence of epitopes common to both Lactobacilli and the host. In fact, molecules reacting with anti-GA1 antibodies were detected among bacterial proteins on Western blotting, indicating a possible occurrence of the carbohydrate structure mimicking GA1 in bacterial proteins.
Key Words: asialo GM1, bacterial receptor, digestive tract, glycoglycerolipids, TLC-immunostaining
Abbreviations: CL, cardiolipin; CMH, ceramide monohexoside; CS, cholesterol sulphate; DG, diacyl glycerol; Hep, L-glycero-D-mannoheptose; FGA1, fucosyl asialo GM1; FGM1, fucosyl GM1; GA1, asialo GM1; PG, phosphatidyl glycerol; Sul, sulphatide
*The nomenclature for glycolipids and gangliosides is based on the recommendations of the IUPAC-IUB Commission on Biochemical Nomenclature [IUPAC-IUB Commission on Biochemical Nomenclature. (1977) The nomenclature of lipids. Eur. J. Biochem. 179, 11–21] and Svennerholm [Svennerholm, L. (1963) Chromatographic separation of human brain gangliosides. J. Neurochem. 10, 613–623], respectively.