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Journal of Biochemistry Advance Access originally published online on August 5, 2009
Journal of Biochemistry 2009 146(5):733-742; doi:10.1093/jb/mvp120
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© The Authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved

Differential Assembly Properties of Escherichia coli FtsZ and Mycobacterium tuberculosis FtsZ: An Analysis Using Divalent Calcium

Richa Jaiswal and Dulal Panda*

School of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Powai, Mumbai – 400076, India

*To whom correspondence should be addressed. Tel: +91-22-2576-7838, Fax: +91-22-2572-3480, E-mail: panda{at}iitb.ac.in

Received July 7, 2009; Accepted July 25, 2009


  Abstract

The assembly of FtsZ is considered to be a fundamental process during the bacterial cytokinesis. We used several complimentary techniques to probe the assembly of recombinant Escherichia coli FtsZ (EcFtsZ) and Mycobacterium tuberculosis FtsZ (MtbFtsZ) proteins in vitro. As documented earlier, EcFtsZ was found to polymerize at much faster rate than MtbFtsZ. Interestingly, we found that MtbFtsZ produced higher sedimentable polymerized mass than that of the EcFtsZ and that MtbFtsZ formed thicker protofilaments than that of the EcFtsZ. The results indicated that the EcFtsZ polymers are more labile than the MtbFtsZ polymers. Further, divalent calcium exerted strikingly different effects on the assembly of EcFtsZ and MtbFtsZ. Divalent calcium strongly enhanced the assembly of EcFtsZ and promoted bundling and stability of the protofilaments. In contrast, it had no detectable effect on the assembly of MtbFtsZ. In vitro, divalent calcium bound to EcFtsZ with much stronger affinity than to MtbFtsZ and significantly affected the secondary structure of EcFtsZ whereas it did not cause any detectable change in the secondary structure of MtbFtsZ. The results suggested that the assembly characteristics of EcFtsZ and MtbFtsZ are different and indicated that the assembly dynamics of these proteins are regulated by different mechanisms.

Key Words: FtsZ assembly, bundling of protofilaments, polymer stability, GTPase activity, calcium

Abbreviations: EcFtsZ, Escherichia coli FtsZ; GTP, guanosine-5'-triphosphate; MtbFtsZ, Mycobacterium tuberculosis FtsZ


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