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J. Biochem, 1967, Vol. 62, No. 3 321-327
© 1967 Japanese Biochemical Society

research-article

Inhibition by Pluramycin A of Nucleic Acid Biosynthesis*

KAZUO NAGAI, HIROSHI YAMAKI, NOBUO TANAKA and HAMAO UMEZAWAK

From the Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo

Pluramycin A, an antitumor and antibacterial antibiotic produced by Streptomyces pluncolorescens, inhibits protein and nucleic acid syntheses by intact cells of E. coli B at the concentration innocuous to energy generation. Synthesis of DNA was most profoundly and that of RNA significantly inhibited by this antibiotic. When total RNA was separated into sRNA, 16S RNA, 23S RNA and mRNA by MAK column or sucrose density gradient centnfugation, all species of RNA were shown to be inhibited at the same level. The results were in accordance with those of shift up and shift down experiments. The inhibition of protein synthesis was chiefly attributed to the secondary effect produced by insufficient supplement of mRNA. The same tendency of inhibition by pluramycin A of DNA, RNA and protein syntheses was observed with HeLa cells in tissue cultures.

*This investigation was supported in whole by Public Health Research Grant CA 05082-06, from the U.S. National Cancer Institute.


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