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J. Biochem, 1968, Vol. 63, No. 2 254-260
© 1968 Japanese Biochemical Society

research-article

Studies on Erythrocyte Glycolysis

VII. Changes of Glycolytic Intermediates in Erythrocytes during Storage in Acid-citrate-dextrose Medium*

HARUMI OHYAMA{dagger}, SHIGEKI MINAKAMI** and HARUHISA YOSHIKAWA

{dagger}From the Department of Radiation Health, National Institute of Radiological Sciences, Chiba abd the Department of Physiological Chemistry and Nutrition, Faculty of Medicine, the University of Tokyo Bunkyo-ku, Tokyo

** Present address: Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka.

1. The changes of glycolytic intermediates and adenine nucleotides in blood were followed during storage in acid-citrate-dextrose (ACD) medium. Until the end of 48 hr storage, hexose 6-phosphates increased gradually, while fructose 1, 6-diphosphate and triose phosphates decreased very rapidly.

2. For the longer period of storage with ACD, hexose 6-phosphates increased during the first week and then decreased, whereas changes of fructose diphosphate and triose phosphates were slight. Monophosphoglycerate, phosphoenopyruvate and pyruvate decreased in the second week and then increased again after the 6th week. 2, 3-Diphosphoglycerate was degradated rapidly whereas ATP decreased more slowly as compared with the rate of degradation of 2, 3-diphosphoglycerate.

3. When ACD blood stored for 6 weeks was incubated with inosine, all the glycolytic intermediates increased remarkably. With addition of both inosine and adenine, ATP was regenerated and hexose 6-phosphate, fructose diphosphate and triose phosphate concentration in the mixture were lower than those in the sample incubated with inosine alone.

4. The glycolytic rate of the stored cells was kept well for 4 weeks and then decreased.

5. The glycolytic activity of the hemolysate from the 16 week-stored red cells was restored to some extent by the addition of both ATP and NAD.

* Dedicated to Dr. Koozoo Kaziro, Professor Emeritus of the Nippon Medical School, on the occasion of his 70th birthday.


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