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J. Biochem, 1968, Vol. 63, No. 2 261-269
© 1968 Japanese Biochemical Society

research-article

On Some Properties of Crystalline Cytochrome c 553 from Petalonia fascia*,**

YASUTOMO SUGIMURA and EIJIRO YAKUSHIJI

From the Department of Biology, Faculty of Science, Toho University Chiba

A c-type cytochrome was obtained in crystalline form from a brown alga Petalonia fascia. The absorption maxima were situated at 273, 317.5 ({delta}-max), 415.5({Upsilon}-max), 521.5(ß-max) and 553 mµ({alpha}-max with a shoulder at 549 mµ in reduced state. The maxima of the difference spectrum (reduced-oxidized) were located at 417, 521.5 and 553 mµ with a shoulder at 549 mµ. The millimolar extinction coefficient at 553 mµ of the ferro-cytochrome was 28.5 mM–1cm–1, and the millimolar extinction coefficient at 549.5 mµ({alpha}-max) of the pyridine ferrohaemochrome from the cytochrome was 37.9 mM–1cm–1. The molecular weight of the cytochrome was calculated from the iron content (0.530%) to be 105x102. The normal oxidation-reduction potential of the cytochrome was determined by equilibration with the ferro-ferricyanide system to be +0.360 V at pH 7.0 and 20°C.

* Dedicated to Dr. Kaziro, Professor Emeritus of the Nippon Medical School, on the occasion of his 70th birthday.

** The study was supported in part by the grant (GM 05871—09) from the National Institutes of Health, U.S.A. and in part by the grant (4093) from the Ministry of Education, Japan.


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