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J. Biochem, 1970, Vol. 67, No. 2 175-184
© 1970 Japanese Biochemical Society

research-article

The Degradation of Ribosomal RNA in E. coli by Mitomycin C and AF-5, Preferential Inhibitors of DNA Synthesis

NOBUO KATO, KINJI OKABAYASHI and DEN'ICHI MIZUNO

Facutly of Pharmaceutical Sciences, University of Tokyo Hongo, Tokyo

When exponentially growing cells of E. coli were treated with Mitomycin C (MC) or Panfuran (AF-5), the following results were obtained.

  1. Simultaneous synthesis and degradation, i.e., a rapid turnover of RNA was observed in the presence of MC or AF-5, whereas DNA remained unaffected during the treatment.
  2. The degraded RNA was shown to be the ribosomal RNA by methylated albumin kieselgur column chromatography.
  3. The total activity of RNase I [EC 2.7.7.16 [EC] [EC] ] in the treated cells was not different from that in the control cells. Ribosomes prepared from the drug treated cells were degraded at the same rate as those from control cells in the presence of such agent as EDTA, KC1 or NaH2PO4.
  4. By the treatment of E. coli with the drug, several new ribosomal particles appeared in the cell.


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