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J. Biochem, 1970, Vol. 67, No. 2 237-248
© 1970 Japanese Biochemical Society

research-article

Isolation and Characterization of Nucleoprotein, "Nuclear Fraction," from Bacillus subtilis*

MASAO TAKEUCHI and AKIRA TSUGITA**

Department of Molecular Genetics, Medical School, Osaka University Osaka

The nuclear fraction was isolated from B. subtilis. It is composed of DNA, RNA, and protein in the ratio of 1: 0.2–0.3: 0.8–0.9. The physicochemical properties of the nuclear fraction were also studied.

The nuclear fraction was dissociated by a CsCl density gradient centrifugation into DNA with a portion of RNA, free RNA, protein with another portion of RNA, and free protein. The RNA fractions thus separated had different sedimentation coefficients.

The amino acid composition of the protein in the nuclear fraction was analyzed. From the analysis, the protein was found to be either a neutral or an acidic protein, i.e., different from the nuclear protein in animal cells(histone), which have been known to be basic.

When the nuclear protein was mixed with the nuclear DNA, a protein-DNA complex was formed. The recombined complex behaved in the same manner as the original nuclear fraction with regard to a melting profile and Sephadex column chromatography.

* This work was supported by a grant from Japanese Ministry of education and a grant No. 5 ROI GM-10982 from the U.S. Public Health Service.

** Present address: Research Institute for Microbial Diseases, Osaka University, Osaka.


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