Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by ISHIKAWA, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ISHIKAWA, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1970, Vol. 67, No. 2 297-312
© 1970 Japanese Biochemical Society

research-article

Properties of an Oxidative Phosphorylation System Reconstituted from Coupling Factors in Micrococcus lysodeikticus*

SHINJI ISHIKAWA

Biochemistry Division, Institute of Brain Research, Faculty of Medicine, University of Tokyo Bunkyo-ku, Tokyo

  1. A system for oxidative phosphorylation was reconstituted from three components of Micrococcus lysodeikticus: membrane fragments, coupling factor cF and coupling factor Ft, or from membrane fragments and either one of these coupling factor.
  2. ATPase [EC 3.6.1.3 [EC] [EC] ] activity which was sensitive to the cation translocating uncoupler, gramicidin A was also reconstituted with this system. It was suggested that the reconstitution of this gramicidin sensitive ATPase indicated the partial or total reconstitution of energy transfer starting from ATP. The effects of other antibiotics affecting ion transport across the inner mitochondrial membrane were also tested on the reconstituted phosphorylation and ATPase systems.
  3. The effects of trypsin [EC 3.4.4.4 [EC] [EC] ] on the isolated coupling factors and on the reconstituted system were examined. Only the isolated factors were highly susceptible to trypsin, whereas the reconstituted system was resistant in the presence of divalent cations.
  4. The results were compared with those on reconstituted mitochondrial and chloroplast energy transfer systems, and with other ATPase systems of the bacterial membrane.

* This work was presented at the VIIth International Congress of Biochemistry in Tokyo, August 1967.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.