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J. Biochem, 1970, Vol. 68, No. 1 81-89
© 1970 Japanese Biochemical Society

research-article

Immunochemical Studies on Antigenic Substances Produced by Intergroup-Hybrids of Salmonella

Naohisa KOCHIBE

The Department of Legal Medicine, School of Medicine, Gunma University Maehashi

Lipopolysaccharides (LPS) * and haptenic polysaccharides produced by intergroup recombinants of Salmonella were investigated.

1. The O-antigenic activities and contents of specific sugars were much less in LPS preparations from SR type recombinants than in those from the parent (S form). LPS of R-type recombinants was entirely devoid of the parental activities and sugars of the antigens.

2. Recombinants between S. abony (donor) and S. milwaukee (recipient) produced defective LPS, lacking glucosamine at the terminal position of the R-core.

3. An antigenicity common to R recombinants was demonstrated in the SR-LPS of the progenies of a cross between S. riogrande (donor) and S. poona (recipient), suggesting that the R-core is exposed and can react with R-antibody owing to reduction in the number of O-side chains in the macromolecule.

4. Haptenic polysaccharides with O-antigenic specificities of the recipients were found in extracts of the SR recombinants. These substances were isolated and shown immunologically and biochemically to have polymerized O-side chains.

*The following abbreviations are used: LPS, lipopolysaccharide; S, smooth; SR, semirough; R, rough; Hep, aldoheptose; KDO, 2-keto-3-deoxyoctonate; Glc, glucose; Gal, galactose; Man, mannose; Fuc, fucose; Rha, rhamnose; G1cN, glucosamine; Ga1N, galactosamine; Abe, abequose.


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