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J. Biochem, 1974, Vol. 76, No. 4 745-753
© 1974 Japanese Biochemical Society

research-article

The Role of Tryptophan-62 in the Enzymic Reaction of Lysozyme

Taiji IMOTO, Masanori FUJIMOTO and Kazuyoshi YAGISHITA

Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University Yamaguchi 753

Trp-62 oxidized lysozyme was purified by affinity chromatography. The enzymic activities of Trp-62 oxidized lysozyme toward glycol chitin and bacterial cells were examined and the Vmax values were found to be 8±1 and 29±4% of those of native lysozyme [EC 3.2.1.17 [EC] ], respectively.

Not only substrate binding but also the catalytic importance of Trp-62 in lysozyme action was discussed. A cooperative effect of Trp-62 and lysyl amino group(s) in lytic action can explain the higher Vmax value in lysis.


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