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J. Biochem, 1974, Vol. 76, No. 4 783-789
© 1974 Japanese Biochemical Society

research-article

Microdetermination of Individual Neutral and Amino Sugars and N-Acetylneuraminic Acid in Complex Saccharides*

Seiichi TAKASAKI and Akira KOBATA

Department of Biochemistry, Kobe University School of Medicine Kusunoki-cho, Ikuta-ku, Kobe 650

A very sensitive method for the quantitative determination of individual neutral and amino sugars and N-acetylneuraminic acid in complex saccharides has been developed. The usefulness of this method was confirmed by applying it to the analysis of several milk oligosaccharides of known structure.

*This work has been supported in part by Research Grants from the Jane Coffin Childs Memorial Fund for Medical Research, the Naito Foundation, and the Scientific Research Fund (1973–1974) of the Ministry of Education of Japan.


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