Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by INOUYE, Y.
Right arrow Articles by OSAWA, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by INOUYE, Y.
Right arrow Articles by OSAWA, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1974, Vol. 76, No. 4 791-799
© 1974 Japanese Biochemical Society

research-article

Conversion of Glucose and Galactose to Lipids by Normal and Phytohemagglutinin-stimulated Lymphocytes

Yoshio INOUYE*, Shizuo HANDA** and Toshiaki OSAWA*

*Division of Chemical Toxicology and Immunochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo Bunkyo-ku, Tokyo 113
**Department of Biochemistry, Faculty of Medicine, University of Tokyo Bunkyo-ku, Tokyo 113

The lipid metabolism of human lymphocytes after stimulation with Phaseolus vulgaris hemagglutinin (PHA) for 4 days was investigated by means of [6-3H]glucose and [1-3H]galactose incorporation. In the case of [6-3H]glucose incorporation, most of the radioactivity (70–80%) is incorporated into neutral lipids, especially into triglycerides. However, the incorporation of radioactivity into polar lipids, namely phospholipids and sphingoglycolipids, is somewhat enhanced in PHA-stimulated lymphocytes compared with normal lymphocytes. In contrast, only 35–40% of the incorporated radioactivity was attributable to neutral lipids in the case of [1-3H]galactose incorporation and more than 40% of the incorporated radioactivity was found in sphingoglycolipids. Among the sphingoglycolipids, the incorporation into ceramide trihexoside was most accelerated, with the following order of incorporation into other sphingoglycolipids: glucosyl ceramide > ceramide dihexoside > globoside I. Significant incorporation of radioactivity into galactosyl ceramide was not observed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.