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J. Biochem, 1974, Vol. 76, No. 4 901-904
© 1974 Japanese Biochemical Society

other

A Simple Method for Extraction and Partial Purification of Chlorophyll from Plant Material, Using Dioxane

Keiji IRIYAMA*, Nagao OGURA** and Atusi TAKAMIYA***

*Division 1, Research Institute for Polymers and Textiles Kanagawa-ku, Yokohama, Kanagawa 221
**Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University Matsudo, Chiba 271
***Department of Biology, Faculty of Science, Toho University, Narashino Chiba 275

A convenient laboratory method for partial purification of chlorophylls using dioxane is described. Chlorophyll in crude extracts with 90%-methanol or 80%-acetone was mixed with dioxane and then precipitated by drop-wise addition of water. Chlorophyll is precipitated in spherocrystalline form, leaving the bulk of carotenoids in solution. This is an efficient method for preparing chlorophyll from various plant materials, including higher plants and algae as well as photosynthetic bacteria (bacteriochlorophyll).


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