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J. Biochem, 1974, Vol. 76, No. 6 1359-1361
© 1974 Japanese Biochemical Society

research-article

Tentative Amino Acid Sequence of Ribonuclease U11

Junji HASHIMOTO2 and Kenji TAKAHASHI3

Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo Bunkyo-ku, Tokyo 113

A tentative amino acid sequence of RNase U1 [EC 3.1.4.8 [EC] ] was derived by aligning tryptic and chymotryptic peptides obtained from the oxidized protein on the basis of maximal homology with the sequence of RNase T1 and with the aid of overlapping peptides. The sequence thus derived is different from that of RNase T1 in about 60% of the total residues. However, it was found to contain the amino acid sequences corresponding to those in RNase T1 involving His-40 and -92, Glu-58, and Arg-77 which have been implicated in the active site of RNase T1. These results strongly support the importance in the activity of these residues in RNase T1 and of the corresponding residues in RNase U1.

1This study was supported in part by grants from the Minstry of Education of Japan and the Naito Foundation (Naito grant for 1969).

2present address: Institute for Plant Virus Research, Aoba-cho, Chiba 280.

3Present address: Department of Biochemistry, Primate Research Institute, Kyoto University, Inuyama, Aichi 484. To whome all correspondence should be addressed.


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