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J. Biochem, 1976, Vol. 79, No. 6 1123-1133
© 1976 Japanese Biochemical Society

research-article

Properties of Liposomal Membranes Containing Lysolecithin

Takayuki KITAGAWA1, Keizo INOUE2 and Shoshichi NOJIMA2

Department of Chemistry, National Institute of Health Kamiosaki, Shinagawa-ku, Tokyo 142

Liposomes have been prepared with lysolecithin (l-acyl-sn-3-glycerylphosphorylcholine), egg lecithin (3-sn-phosphatidylcholine), dicetyl phosphate, and cholesterol. The ability to function as a barrier to the diffusion of glucose marker and the sensitivities of the liposomes to hypotonic treatment and other reagents which modified the permeability were examined. Generally, lysolecithin incorporation decreased the effectiveness of the membranes as a barrier to glucose and made the membranes more "osmotically fragile." Cholesterol incorporation counteracted the effect of incorporated lysolecithin. The more cholesterol incorporated into liposomes, the more lysolecthin could be incorporated into the membrane without loss of function as a barrier. With more than 50 mole% of cholesterol, lysolecithin alone could form membranes which were practically impermeable to glucose. The hemolytic activity of lysolecithin was affected by mixing with various lecithins or cholesterol. Liposomes containing lysolecithin, which have the ability to trap glucose marker, showed poor hemolytic activity, while lipid micelles with lysolecithin (which could trap little glucose) showed almost the same hemolytic activity as lysolecithin itself. There seems to be a close correlation between hemolytic activity and barrier function of lipid micelles.

1Present address: Department of Virology, Institute of Medical Sciences, University of Tokyo, Minato-ku, Tokyo 108.

2Present address: Department of Health Chemistry, Faculty of Pharamaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113.


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