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J. Biochem, 1976, Vol. 79, No. 6 1183-1196
© 1976 Japanese Biochemical Society

research-article

Fragmentation of Rat Serum Albumin by Cyanogen Bromide Cleavage and the Amino Acid Sequences of Four Fragments1

Satoko ISEMURA and Tokuji IKENAKA

Department of Biochemistry, Niigata University School of Medicine Niigata, Niigata 951

Rat serum albumin was cleaved into seven peptides by cyanogen bromide treatment followed by reduction and carboxymethylation. The amino acid composition and the N-terminus of each peptide and the amino acid sequences of four peptides were determined by conventional methods. An alignment of these peptides in the original protein was proposed in the light of the established sequences of bovine and human plasma albumin.

1This study was supported in part by a scientific research grant from the Ministry of Education, Science and Culture, of Japan.


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