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J. Biochem, 1976, Vol. 79, No. 6 1197-1200
© 1976 Japanese Biochemical Society

research-article

Fluorometric Method for Assay of Kallikrein-like Arginine Esterases

Yoshifumi MATSUDA, Hiroshi MORIYA, Chiaki MORIWAKI, Yukio FUJIMOTO1 and Mihoko MATSUDA2

Laboratory of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo Ichigaya, Shinjuku-ku, Tokyo 162

The fluorometric method of Nash for determination of methanol was applied in assay of kallikrein-like arginine esterases using N-{alpha}-tosyl-L-arginine methyl ester (TAME) as substrate. The results obtained using this method corresponded well with those obtained in the usual colorimetric methods using hydroxamate, chromotropic acid, and 3-methyl-2-benzothiazolone hydrazone and the present method was more sensitive than these colorimetric methods.

1Present address: Hokkaido Institute of Pharmaceutical Sciences, 62 Katsuragaoka-cho, Otaru-shi, Hokkaido 047-02.

2Present address: Laboratory of Racing Chemistry, Kamiyoyga, Setagaya-ku, Tokyo 158.


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