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J. Biochem, 1976, Vol. 79, No. 6 1297-1299
© 1976 Japanese Biochemical Society

research-article

A Simple Fluorometric Assay for Type B Monoamine Oxidase Activity in Rat Tissues

Osamu SUZUKI, Etsuko NOGUCHI and Kunio YAGI

Institute of Biochemistry, Faculty of Medicine, University of Nagoya Showa-ku, Nagoya, Aichi 466

A simple fluorometric assay for monoamine oxidase (MAO) [EC 1.4.3.4 [EC] ] activity towards ß-phenylethylamine (PEA) was devised. The procedure consists in measuring the disappearance of PEA fluorometrically. The disappearance of PEA was completely inhibited by pargyline, a potent inhibitor of MAO. MAO activity for PEA was linear with 10 mg to 100 mg of liver tissue in 3 ml of reaction mixture for up to 90 min of incubation. Using this method, the Vmax values and the apparent Km values of MAO for PEA in several rat tissues were determined, and compared with those for benzylamine and 5-hydroxytryptamine (5-HT).


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