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J. Biochem, 1976, Vol. 79, No. 6 1323-1330
© 1976 Japanese Biochemical Society

research-article

Effect of Priming Doses of Chemically Modified Antigen on Helper Activity

Shoichi NAKASHIMA, Nobuaki TAKE, Hirohito HAYASHI and Masanori MAZAKI

Department of Biochemistry, Drug Research Institute, Toyama University Gofuku, Toyama, Toyama 930

Mice primed with chemically modified bacterial {alpha}-amylase (B{alpha}A) [EC 3.2.1.1 [EC] {alpha}-amylase, B. subtilis], which was neither cross-reactive with anti-B{alpha} A antibody nor able to induce a humoral anti-B{alpha}-A antibody response, developed enhanced responses to a subsequent challenge with native B{alpha}A (Nakashima et al. (1974) J. Biochem. 76, 349 — 357). The present studies were designed to examine the relationship of priming doses of B{alpha}A derivatives to the level of enhancement of the helper activity.

Increasing the priming dose of modified antigens resulted in a greater degree of helper cell response until the maximal level of enhancement was reached. When injections for priming and challenge were given intraperitoneally, priming doses of D-B{alpha}A, M-B{alpha}A, and RM-B{alpha}A required for the maximal enhancement of helper activity were about 15, 50, and 15 µg, respectively. Further increase in the priming dose, conversely, resulted in suppressin of the enhanced helper activity, irrespective of whether the time interval between priming and challenge was 10 or 28 days. Suppression of the enhanced helper activity upon excessive dose priming with modified B{alpha}A derivatives was not specific for the anti-B{alpha}A antibody response. On the basis of these results it is suggested that this phenomenon of suppression might be partly accounted for by the regulatory mechanism functioning in antigenic competition.


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