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J. Biochem, 1976, Vol. 79, No. 6 1357-1364
© 1976 Japanese Biochemical Society

research-article

Roles of Nucleoside Triphosphates in Microtubule Assembly

Takaaki KOBAYASHI* and Takashi SIMIZU**

*Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo Hongo, Bunkyo-ku, Tokyo 113
**Division 1, Research Institute of Polymers and Textiles Sawatari, Kanagawa-ku, Yokohama Kanagawa, 221

Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35° with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruvate and pyruvate kinase [EC 2, 7.1.40] also induced polymerization. Depolymerization of microtubules in the GTP-reassembly buffer yielded GTPETNGTP, which was capable of polymerizing into microtubules even in the absence of free GTP. In the presence of 4 M glycerol, GDPETNGTP assembled into microtubules with no change in the bound nucleotides.


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