Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Request Permissions
Google Scholar
Right arrow Articles by OHKUMA, S.
Right arrow Articles by YASUDA, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by OHKUMA, S.
Right arrow Articles by YASUDA, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

J. Biochem, 1976, Vol. 79, No. 6 1365-1376
© 1976 Japanese Biochemical Society

research-article

Synthesis of Apoferritin in Mouse Peritoneal Macrophages Characterization of 20 S Particles1

Shoji OHKUMA*,2, Hiroshi NOGUCHI*,3, Fumio AMANO*, Den'ichi MIZUNO* and Tomoyoshi YASUDA**

*Faculty of Pharmaceutical Sciences, University of Tokyo Bunkyo-ku, Tokyo 113
**National Institute of Health Shinagawa-ku, Tokyo 141

Apoferritin particles were found in mouse peritoneal macrophages cultured in vitro. They were found as 20S particles in the "ribosomal fraction" of macrophages labeled with L-[14C]glutamic acid. Possibilities that they were breakdown products of ribosomes or of other well-known contaminants of the ribosomal fraction were excluded because they did not incorporate [5-3H]uridine. They were resistant to RNase and were relatively resistant to detergent.

The antibody against horse spleen apoferritin precipitated about 70% of the particles in the 20S region, judging by measurement of radioactivity. On in vitro incubation with Fe2+ and suitable oxidizing agents the sedimentation coefficient of 80% of the 20S particles changed to about 60S, which corresponds to that of ferritin. SDS-polyacrylamide gel electrophoresis revealed the presence of subunit structures with the same molecular size as that of mouse liver apoferritin. Under the electron microscope, the particles appeared spherical with a relatively uniform diameter of about 130 A.

1This study was supported by a Scientific Research Grant from the Ministry of Education, Science and Culture, of Japan.

2Present address: The Rockefeller University, New York, N.Y. 10021, U.S.A.

3Present address: Sumitomo Chemicals Industry Ltd., Tkarazuka, Hyogo 665.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.