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J. Biochem, 1976, Vol. 79, No. 6 1377-1379
© 1976 Japanese Biochemical Society

other

Reconstruction of Intact {gamma}-Globulin from S-Sulfonated {gamma}-Globulin In Vivo

Yasuhiko MASUHO, Katsuhiko TOMIBE, Kimihiko MATSUZAWA, Tsuneo WATANABE, Sachio ISHIMOTO, Satoru TSUNODA and Teruhisa NOGUCHI

Teijin Institute for Biomedical Research Hino, Tokyo 191

The conversion of the S-surfonate group in a peptide to a disulfide bond has been observed in vitro. This paper reports that the conversion of this group in human S-sulfonated {gamma}-globulin (S-GG) appeared to occur in vivo judging from the change in molecular weight of S-GG observed in the presence of sodium dodecyl sulfate (SDS) and the restoration of hemolytic activity.


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